Q1:How is the mixing time determined during medium preparation? If insoluble particles persist after long-term stirring (over 1 hour), how should they be handled?

Please first ensure that the culture medium is dissolved according to the preparation method provided by the supplier. If the issue persists despite following the instructions, you may contact the culture medium supplier for analysis and resolution.

Q2:Can the Eden series basal medium be used in combination with feed medium 7a/7b?

It is highly recommended to use the combination of the Eden series Basal medium and feeds. The design and development of the Eden feeds medium are based on the Eden Basal medium, and accumulated data suggests that the combination of Eden series media yields superior results. Furthermore, as the formulation is independently developed, it can be fine-tuned to meet a wider range of requirements. Of course, you can also try the combination of 7a7b and the Eden basal medium, then based on the results, determine which combination to use.

Q3:BioEngine offers multiple options for CHO cell culture medium. How do I choose the right one?

BioEngine has developed multiple culture media and various feed combinations to meet the specific requirements of different cell lines and project types, aiming to achieve optimal results. Please contact us for further support and assistance.

Q4:Does BioEngine's Eden series CHO CD medium contain Pluronic F-68 (PF-68)?

The Eden series basal media and Feed A media contain PF-68

Q5:What are the packaging sizes available for the Eden series CHO cell culture medium products?

The Eden series powder basal media are available in 10L, 100L, and 200L sizes, and feeds media are available in 1L, 10L, 20L, and 50L sizes.

Q6:What is F12 in DMEM?

DMEM (Dulbecco's Modified Eagle Medium) is a cell culture medium commonly used for the growth and maintenance of many types of mammalian cells. DMEM/F12 is a 1:1 mixture of DMEM and Ham's F12 media. It is also a commonly used cell culture medium and has been reported to support the growth of a wide variety of mammalian cells, including many types of cancer cells, primary cells, and stem cells. The addition of F12 to DMEM provides additional nutrients and growth factors that are not present in DMEM alone, which can enhance cell growth and viability. BioEngine provides many serum -free cell culture media products in biomedical industry including specific culture media for animal cell and classical media such as DMEM, DMEM/F12, RPMI 1640, etc.

Q7:What is perfusion culture？

Perfusion culture is a type of bioreactor operation used in industrial biotechnology for the continuous cultivation of cells or microorganisms. In a perfusion culture, fresh culture medium is continuously added to the bioreactor while an equal volume of spent medium, containing cells or microorganisms, is continuously removed. This allows for a constant supply of nutrients, oxygen, and other growth factors, while also removing waste products and preventing the accumulation of toxic metabolites.

Perfusion culture is commonly used for the production of high-value biopharmaceuticals, such as monoclonal antibodies, where high cell densities are required to achieve sufficient product yields. In a perfusion culture, cells can be grown at higher densities and for longer periods of time, leading to higher productivity and more efficient use of resources than in traditional batch or fed-batch cultures.

Perfusion culture can be operated in various ways, including tangential flow filtration (TFF), spin filters, and hollow fiber membranes, each with its own advantages and limitations. TFF-based perfusion culture is the most used technique due to its scalability, ease of operation, and ability to maintain consistent cell densities and product quality over time.

Q8:What are the applications of CHO cells?

CHO cells, or Chinese Hamster Ovary cells, are commonly used in biomedical research and biomanufacturing due to their ability to produce large amounts of recombinant proteins. Some of the applications of CHO cells are:

1. Production of biopharmaceuticals: CHO cells are widely used in the production of biopharmaceuticals, such as monoclonal antibodies, growth factors, and hormones. These proteins can be used for the treatment of a range of diseases, including cancer, autoimmune disorders, and diabetes.

2. Drug discovery: CHO cells are often used in drug discovery research to screen and test potential drug candidates. They are particularly useful for testing drugs that target specific receptors or proteins.

3. Vaccine production: CHO cells can be used to produce vaccines, including those for influenza and human papillomavirus (HPV).

4. Genetic engineering: CHO cells can be genetically engineered to produce specific proteins with desired properties. This can be done by introducing genes that encode for the protein of interest into the cells.

5. Toxicology testing: CHO cells are sometimes used in toxicology testing to evaluate the safety of chemicals or drugs. They can be used to test the toxicity of compounds on cells and to study their effects on cellular pathways.

Overall, CHO cells are a valuable tool in biomedicine, and their applications are constantly expanding as new techniques and technologies emerge.

Q9:What are Vero cell lines used for?

Vero cell lines are used for various applications including virus propagation, vaccine production, drug screening, and toxicity testing. The cells are particularly useful for virus propagation due to their susceptibility to a wide range of viruses and ability to grow in serum-free medium. Vero cell are considered a reliable platform for vaccine production. BioEngine provides Pesche series vero serum-free media for high vaccine production.

Q10:What are low serum media?

Low serum media is a cell culture medium that contains a reduced concentration of fetal bovine serum (FBS) or other animal serum, typically 1-5% compared to the standard 10-20% used in traditional cell culture. This type of medium is commonly used for applications such as reducing the influence of serum on experimental results or for culturing cells with low serum requirements. It is also used to reduce the cost of cell culture and to avoid potential complications associated with high serum concentration, such as antibody interference or variability between serum batches. BioEngine provides various serum-free, reduced-serum and classical media for different cells and applications.

Q11:What is a Marc145 cell？

"Marc145 cell is a cell line derived from the kidney of a rhesus macaque monkey in 1981 by S.H. Madin and N.B. Darby. It is an epithelial cell line that can be used for the isolation and propagation of porcine reproductive and respiratory syndrome virus (PRRSV), which is a swine pathogen that causes respiratory and reproductive disorders. Marc145 cell is highly susceptible to infection by PRRSV and can support efficient viral replication and production23. Marc145 cell can also be used for transfection studies, 3D cell culture, cancer research, and bioproduction. BioEngine provides serum-free or reduced-serum medium for Marc145 cell. Please contact us for more information."

Q12:How do I prepare cell culture media from powder?

Detailed protocols and instructions for the preparation and usage of all our cell culture media products are available for download on the BioEngine website or can be obtained by contacting us.

Q13:How can cell transfection be classified?

"Cell transfection is the process of introducing foreign nucleic acids, such as plasmid DNA or siRNA, into cells. There are several ways to classify cell transfection methods, and one possible classification is based on the mechanism of delivery, which can be classified into the following categories:

1. Chemical transfection: This method uses chemical agents to facilitate the uptake of nucleic acids into cells. Lipofection and calcium phosphate transfection are examples of chemical transfection methods.

2. Electroporation: This method uses an electric field to create transient pores in the cell membrane, allowing nucleic acids to enter the cell. Electroporation is commonly used for transfection of difficult-to-transfect cells.

3. Viral transfection: This method involves the use of viral vectors, which are modified viruses that can deliver nucleic acids into cells. Lentiviral and retroviral transfection are examples of viral transfection methods.

4. Physical transfection: This method uses physical forces, such as microinjection or gene gun, to deliver nucleic acids into cells.

5. Biolistic transfection: This method uses high-pressure helium gas to deliver nucleic acids into cells. It is also known as the ""gene gun"" method.

6. Magnetofection: This method involves using magnetic nanoparticles to deliver nucleic acids into cells, which are then targeted to specific regions of the cell by applying a magnetic field.

These methods can also be further classified based on whether they are transient or stable. Transient transfection refers to the temporary expression of the introduced nucleic acids, whereas stable transfection results in the integration of the nucleic acids into the host cell genome, resulting in long-term expression of the introduced genes."